In vitro cytotoxicity of curcuminoids against head and neck cancer HNO97 cell line

Abstract Oral squamous cell carcinoma (OSCC) is a malignant tumour of Head and Neck Cancer (HNC). The recent therapeutic approaches used to treat cancer have adverse side effects. natural agents exhibiting anticancer activities are generally considered robust potential. Curcuminoids, one the major active compounds turmeric herb, as agent for several diseases including cancer. In this study, cytotoxicity curcuminoids was investigated against OSCC line HNO97. Our data showed that significantly inhibits proliferation HNO97 in time dose-dependent manner (IC50=35 μM). Cell cycle analysis demonstrated increased percentage G2/M phase populations treated groups. Treating cells with led shrinking detached cells, which typical appearance apoptotic cells. Moreover, flow cytometry revealed induced apoptosis time-dependent manner. Furthermore, response treatment, comet tails were formed nuclei due induction DNA damage. Curcuminoids treatment reduced colony formation capacity morphological changes. Overall, these findings demonstrate can vitro inhibit HNC metastasis induce apoptosis.